Development of a rapid colorimetric multiplex PCR–reverse line blot for the detection and typing of 14 Chlamydia trachomatis genovars | Zendy

Mónica Molano | Zendy; Sepehr N. Tabrizi | Zendy; Samuel Phillips | Zendy; Jennifer Danielewski | Zendy; Alyssa M. Cornall | Zendy; Servaas A. Morré | Zendy; Suzanne M. Garland | Zendy

Open Access

Development of a rapid colorimetric multiplex PCR–reverse line blot for the detection and typing of 14 Chlamydia trachomatis genovars

Author(s) -

Mónica Molano,

Sepehr N. Tabrizi,

Samuel Phillips,

Jennifer Danielewski,

Alyssa M. Cornall,

Servaas A. Morré,

Suzanne M. Garland

Publication year - 2018

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.000836

Subject(s) - chlamydia trachomatis , lymphogranuloma venereum , trachoma , multiplex , biology , microbiology and biotechnology , virology , typing , sexually transmitted disease , multiplex polymerase chain reaction , polymerase chain reaction , medicine , gene , genetics , syphilis , pathology , human immunodeficiency virus (hiv)

Chlamydia trachomatis is responsible for trachoma-associated blindness as well as the most common sexually transmitted bacterial infection worldwide, although the genovars for the former are typically A-C, whilst for the latter they are D-K and for the uncommon infection lymphogranuloma venereum they are L1-3. Nucleotide variations within the ompA gene facilitate the identification of C. trachomatis genovars. This study describes a colorimetric multiplex PCR/RLB typing assay (mPCR-RLB) directed to the VD2 region of the ompA gene for general C. trachomatis positivity and the identification of 14 individual C. trachomatis genovars.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research