Open AccessDevelopment of a rapid colorimetric multiplex PCR–reverse line blot for the detection and typing of 14 Chlamydia trachomatis genovars
Author(s) -
Mónica Molano,
Sepehr N. Tabrizi,
Samuel Phillips,
Jennifer Danielewski,
Alyssa M. Cornall,
Servaas A. Morré,
Suzanne M. Garland
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000836
Subject(s) - chlamydia trachomatis , lymphogranuloma venereum , trachoma , multiplex , biology , microbiology and biotechnology , virology , typing , sexually transmitted disease , multiplex polymerase chain reaction , polymerase chain reaction , medicine , gene , genetics , syphilis , pathology , human immunodeficiency virus (hiv)
Chlamydia trachomatis is responsible for trachoma-associated blindness as well as the most common sexually transmitted bacterial infection worldwide, although the genovars for the former are typically A-C, whilst for the latter they are D-K and for the uncommon infection lymphogranuloma venereum they are L1-3. Nucleotide variations within the ompA gene facilitate the identification of C. trachomatis genovars. This study describes a colorimetric multiplex PCR/RLB typing assay (mPCR-RLB) directed to the VD2 region of the ompA gene for general C. trachomatis positivity and the identification of 14 individual C. trachomatis genovars.