Open AccessIntranasal coinfection model allows for assessment of protein vaccines against nontypeable Haemophilus influenzae in mice
Author(s) -
Lea Michel,
Ravinder Kaur,
Mark Zavorin,
Karin S. Pryharski,
M. Nadeem Khan,
Ciara LaClair,
Meghan O'Neil,
Qing Xu,
Michael E. Pichichero
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000827
Subject(s) - haemophilus influenzae , coinfection , streptococcus pneumoniae , immunology , medicine , pneumonia , microbiology and biotechnology , nasal administration , vaccination , antibiotics , virology , haemophilus , virus , biology , bacteria , genetics
Nontypeable Haemophilus influenzae (NTHi) is a commensal in the human nasopharynx and the cause of pneumonia, meningitis, sinusitis, acute exacerbations of chronic obstructive pulmonary disease and acute otitis media (AOM). AOM is the most common ailment for which antibiotics are prescribed in the United States. With the emergence of new strains of antibiotic-resistant bacteria, finding an effective and broad coverage vaccine to protect against AOM-causing pathogens has become a priority. Mouse models are a cost-effective and efficient way to help determine vaccine efficacy. Here, we describe an NTHi AOM model in C57BL/6J mice, which also utilizes a mouse-adapted H1N1 influenza virus to mimic human coinfection.