Open AccessGenotyping based on thermal denaturation of amplification products identifies species of the Mycobacterium tuberculosis complex
Author(s) -
Mariel Idalid Domínguez-Zepahua,
Socorro Hernández-Arteaga,
Rubén LópezRevilla
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000816
Subject(s) - genotyping , genotype , mycobacterium tuberculosis , biology , mycobacterium tuberculosis complex , mycobacterium , 16s ribosomal rna , mycobacterium bovis , polymerase chain reaction , microbiology and biotechnology , amplicon , tuberculosis , genetics , bacteria , medicine , gene , pathology
To develop a fast and inexpensive genotyping assay to identify the Mycobacterium tuberculosis complex (MTC) species most prevalent in human tuberculosis (TB), based on the thermal denaturation profiles of PCR products from mycobacterial 16S rDNA and three MTC genomic regions of difference (RD).