Open AccessDevelopment of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species
Author(s) -
Suat Moi Puah,
Wei Ching Khor,
Boon Pin Kee,
Ji Tan,
S. D. Puthucheary,
Kek Heng Chua
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000796
Subject(s) - biology , restriction fragment length polymorphism , restriction enzyme , genotype , aeromonas , in silico , microbiology and biotechnology , polymerase chain reaction , terminal restriction fragment length polymorphism , aeromonas hydrophila , genetics , gene , bacteria
The taxonomy of Aeromonas keeps expanding and their identification remains problematic due to their phenotypic and genotypic heterogeneity. In this study, we aimed to develop a rapid and reliable polymerase chain reaction-restriction fragment length polymorphism assay targeting the rpoD gene to enable the differentiation of aeromonads into 27 distinct species using microfluidic capillary electrophoresis.