Development of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species | Zendy

Suat Moi Puah | Zendy; Wei Ching Khor | Zendy; Boon Pin Kee | Zendy; Jin Ai Mary Anne Tan | Zendy; Savithri D. Puthucheary | Zendy; Kek Heng Chua | Zendy

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Development of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species

Author(s) -

Suat Moi Puah,

Wei Ching Khor,

Boon Pin Kee,

Jin Ai Mary Anne Tan,

Savithri D. Puthucheary,

Kek Heng Chua

Publication year - 2018

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.000796

Subject(s) - biology , restriction fragment length polymorphism , restriction enzyme , genotype , aeromonas , in silico , microbiology and biotechnology , polymerase chain reaction , terminal restriction fragment length polymorphism , aeromonas hydrophila , genetics , gene , bacteria

The taxonomy of Aeromonas keeps expanding and their identification remains problematic due to their phenotypic and genotypic heterogeneity. In this study, we aimed to develop a rapid and reliable polymerase chain reaction-restriction fragment length polymorphism assay targeting the rpoD gene to enable the differentiation of aeromonads into 27 distinct species using microfluidic capillary electrophoresis.

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