Open AccessNovel multiplex PCR assay for identification and subtyping of enteroinvasive Escherichia coli and differentiation from Shigella based on target genes selected by comparative genomics
Author(s) -
Rajat Dhakal,
Qinning Wang,
Ruiting Lan,
Peter Howard,
Vitali Sintchenko
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000784
Subject(s) - subtyping , biology , shigella , multiplex polymerase chain reaction , gene , identification (biology) , escherichia coli , computational biology , genetics , genomics , multiplex , comparative genomics , polymerase chain reaction , microbiology and biotechnology , genome , computer science , programming language , botany
Both Shigella and enteroinvasive Escherichia coli (EIEC) can cause enterocolitis, but they have a distinct epidemiology and public health relevance. Current culture-independent testing (CIT) methods to identify Shigella in faecal samples rely on the ipaH gene as the target, which is also found in EIEC genomes. The aim of this study was to design an assay that can identify EIEC in cultures from CIT ipaH-positive samples.