Open AccessDetection of Coxiella burnetii in heart valve sections by fluorescence in situ hybridization
Author(s) -
Karin Aistleitner,
Rima Jeske,
Roman Wölfel,
Alexandra Wießner,
Judith Kikhney,
Annette Moter,
Kilian Stoecker
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000704
Subject(s) - coxiella burnetii , q fever , fluorescence in situ hybridization , in situ hybridization , in situ , biology , virology , chemistry , gene , genetics , gene expression , chromosome , organic chemistry
Infective endocarditis is a severe and potentially fatal disease. Nearly a third of all cases remain culture-negative, making a targeted and effective antibiotic therapy of patients challenging. In the past years, fluorescence in situ hybridization (FISH) has proven its value for the diagnosis of infective endocarditis, particularly when it is caused by fastidious bacteria. To increase the number of infective endocarditis causing agents, which can be identified by FISH, we designed and optimized a FISH-probe for the specific detection of Coxiella burnetii in heart valve tissue.