Open AccessRapid discrimination of Staphylococcus epidermidis genotypes in a routine clinical microbiological laboratory using single nucleotide polymorphisms in housekeeping genes
Author(s) -
Kjersti Wik Larssen,
Anne Nor,
Kåre Bergh
Publication year - 2018
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.000663
Subject(s) - staphylococcus epidermidis , housekeeping gene , biology , genotype , genotyping , multilocus sequence typing , single nucleotide polymorphism , amplicon , outbreak , microbiology and biotechnology , genetics , virology , polymerase chain reaction , gene , staphylococcus aureus , bacteria , gene expression
Staphylococcus epidermidis colonies often display several morphologies and antimicrobial susceptibility patterns when cultured from device-related infections, and may represent one or multiple genotypes. Genotyping may be helpful in the clinical interpretation, but is time consuming and expensive. We wanted to establish a method for rapid discrimination of S. epidermidis genotypes for use in a routine microbiology laboratory.