Open AccessFirst isolation and whole-genome characterization of a G9P[14] rotavirus strain from a diarrheic child in Egypt
Author(s) -
Ashraf R.S. Shoeib,
Daniel E. Velasquez Portocarrero,
Yuhuan Wang,
Baoming Jiang
Publication year - 2020
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/jgv.0.001455
Subject(s) - reassortment , rotavirus , biology , genotype , strain (injury) , virology , phylogenetic tree , genome , virus , genetics , gene , infectious disease (medical specialty) , disease , covid-19 , medicine , pathology , anatomy
An unusual group A rotavirus (RVA) strain (RVA/Human-tc/EGY/AS997/2012/G9[14]) was isolated for the first time in a faecal sample from a 6-month-old child who was hospitalized for treatment of acute gastroenteritis in Egypt in 2012. Whole-genome analysis showed that the strain AS997 had a unique genotype constellation: G9-P[14]-I2-R2-C2-M2-A11-N2-T1-E2-H1. Phylogenetic analysis indicated that the strain AS997 had the consensus P[14] genotype constellation with the G9, T1 and H1 reassortment. This suggests either a mixed gene configuration originated from a human Wa-like strain and a P[14]-containing animal virus, or that this P[14] could have been acquired via reassortment of human strains only. The study shows the possible roles of interspecies transmission and multiple reassortment events leading to the generation of novel rotavirus genotypes and underlines the importance of whole-genome characterization of rotavirus strains in surveillance studies.