Open AccessA single amino acid in coat protein of Pepper mild mottle virus determines its subcellular localization and the chlorosis symptom on leaves of pepper
Author(s) -
Kelei Han,
Hongying Zheng,
Mengfei Ji,
Weijun Cui,
Shuzhen Hu,
Jiejun Peng,
Jinping Zhao,
Yuwen Lu,
Lin Lin,
Yong Liu,
Jianping Chen,
Fei Yan
Publication year - 2020
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/jgv.0.001398
Subject(s) - chlorosis , biology , subcellular localization , pepper , virology , mottle , tobamovirus , plant virus , complementary dna , coat protein , virus , rna , botany , horticulture , gene , genetics
Pepper mild mottle virus (PMMoV) causes serious economic losses in pepper production in China. In a survey for viral diseases on pepper, two PMMoV isolates (named PMMoV-ZJ1 and PMMoV-ZJ2) were identified with different symptoms in Zhejiang province. Sequence alignment analysis suggested there were only four amino acid differences between the isolates: Val262Gly, Ile629Met and Ala1164Thr in the replicase, and Asp20Asn in the coat protein. Infectious cDNA clones of both isolates were constructed and shown to cause distinctive symptoms. Chlorosis symptoms appeared only on PMMoV-ZJ2-infected plants and the Asp20Asn substitution in the CP was shown to be responsible. Confocal assays revealed that the subcellular localization pattern of the two CPs was different, CP 20Asp was mainly located at the cell periphery, whereas most CP 20Asn located in the chloroplast. Thus, a single amino acid in the CP determined the chlorosis symptom, accompanied by an altered subcellular localization.