Open AccessThe Ntail region of nucleocapsid protein is associated with the pathogenicity of pigeon paramyxovirus type 1 in chickens
Author(s) -
Yang Song,
Pei Yu,
Yanling Yang,
Jia Xue,
Guozhong Zhang
Publication year - 2019
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/jgv.0.001264
Subject(s) - biology , virulence , virology , pathogenicity , virus , nucleoprotein , gene , genotype , strain (injury) , genome , genetics , microbiology and biotechnology , anatomy
The nucleoprotein (NP) of pigeon paramyxovirus type 1 (PPMV-1) and other paramyxoviruses plays an important role in virus proliferation. A previous study found that NP is associated with the low pathogenicity of PPMV-1 strains in chickens. Here, we investigated which domain of NP is responsible for regulating the pathogenicity of PPMV-1. We found that the Ntail sequences were more diverse for different viral genotypes compared to Ncore sequences. The chimeric rBJ-SG10Ntail strain caused more severe clinical symptoms than the parental rBJ strain, increased the viral copy number in sampled tissues and induced higher IFN-γ gene expression. This demonstrated that the Ntail sequence plays a role in regulating viral virulence. These findings increase our understanding of the Ntail of NP protein and the virulence factors associated with PPMV-1.