Open AccessPhotodynamic inactivation of Bovine herpesvirus type 1 (BoHV-1) by porphyrins
Author(s) -
Amanda Vargas Teles,
Taise M. A. Oliveira,
Fábio Castro Bezerra,
Laís Alonso,
Antônio Alonso,
Iouri Borissevitch,
Pablo José Gonçalves,
Guilherme Rocha Lino de Souza
Publication year - 2018
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/jgv.0.001121
Subject(s) - porphyrin , cationic polymerization , photodynamic therapy , photochemistry , lipophilicity , zinc , virus , biology , virology , chemistry , biochemistry , polymer chemistry , organic chemistry
In this work, the photodynamic efficiency of anionic meso-tetrakis sulfonophenyl (TPPS4), cationic meso-tetrakis methylpyridiniumyl (TMPyP) and their zinc complexes (ZnTPPS4 and ZnTMPyP) in the inactivation of Bovine herpesvirus type 1 (BoHV-1) was evaluated. At a non-cytotoxic concentration, all porphyrins showed significant antiviral activity after irradiation using a halogen lamp. The efficiency of the cationic porphyrins was higher than that of the anionic ones. Porphyrin complexation with zinc increases its lipophilicity and the number of absorbed photons, dramatically reducing the time for complete virus inactivation. The high superposition of the compound optical absorption and light source emission spectra played a key role in the virus inactivation efficiency. The results demonstrated the high effectivity of the photodynamic inactivation of BoHV-1. This method can be used as an auxiliary in the treatment of disorders attributed to BoHV-1 infection, and the porphyrins are promising photosensitizers for this application.