Open AccessMotilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England
Author(s) -
Matthew Kelbrick,
Raeid M. M. Abed,
André Antunes
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004763
Subject(s) - biology , halophile , phylogenetic tree , 16s ribosomal rna , genomic dna , botany , brine , phylogenetics , strain (injury) , genus , bacteria , dna , genetics , gene , physics , thermodynamics , anatomy
A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20 T ) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to Motilimonas eburnea (98.30 %), followed by Motilimonas pumila (96.62 %), the two currently described species within the genus Motilimonas . Strain MKS20 T forms white-beige-pigmented colonies and grows optimally at 28-30 °C, in 1-3 % (w/v) NaCl and at pH 7-7.5. The strain was facultatively anaerobic and showed a broader range of carbohydrate use than other species in the genus Motilimonas . Q-8 was the sole respiratory quinone and the major fatty acids (>10 %) were summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ) and C 16 : 0 . The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyglycerol and several unidentified lipids. The G+C content of the genomic DNA was 44.2 mol%. Average nucleotide identity and DNA-DNA hybridization data were consistent with assignment to a separate species. Based on the phylogenetic and genomic-based analyses, as well as physiological and biochemical characteristics, we propose that strain MKS20 T (=DSM 109936 T , MCCC 1K04071 T ) represents a new species of the genus Motilimonas , with the name Motilimonas cestriensis sp. nov.