Open AccessNonlabens ponticola sp. nov., isolated from seawater and reclassification of Nonlabens sediminis as a later heterotypic synonym of Nonlabens tegetincola
Author(s) -
Ye Lin Seo,
Jaejoon Jung,
Chi-une Song,
Yong Hyun Kwon,
Hye Su Jung,
Seongil Eyun,
Che Ok Jeon
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004603
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , bacteroidetes , bacteria , genomic dna , microbiology and biotechnology , gene , biochemistry , genetics , anatomy
A Gram-stain-negative, orange-pigmented and strictly aerobic bacterium, designated strain MJ115 T , was isolated from seawater in Pohang, South Korea. Cells were non-motile rods and showed positive reactions for catalase and oxidase tests. Growth of strain MJ115 T was observed at 4–35 °C (optimum, 30 °C), pH 6.0–7.0 (optimum, pH 6.5) and in the presence of 0–8.0 % (w/v) NaCl (optimum, 2.0%). Strain MJ115 T contained iso-C 15 : 0 , anteiso-C 15 : 0 , anteiso-C 17 : 1 ω 9 c, C 17 : 0 2-OH, iso-C 16 : 0 3-OH, iso-C 17 : 0 3-OH and summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) as major cellular fatty acids and menaquinone-6 as the major respiratory quinone. Phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids were detected as major polar lipids. The G+C content of the genomic DNA was 40.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ115 T formed a phyletic lineage with Nonlabens marinus S1-08 T , Nonlabens agnitus JC2678 T and Nonlabens antarcticus AKS 622 T within the genus Nonlabens . Strain MJ115 T was also most closely related to N. marinus S1-08 T , N. agnitus JC2678 T and N. antarcticus AKS 622 T with 96.5, 96.4 and 96.0 % 16S rRNA sequence similarities, respectively. Here it is proposed that strain MJ115 T represents a new species of the genus Nonlabens , for which the name Nonlabens ponticola sp. nov. is proposed. The type strain is MJ115 T (=KCTC 72237 T =NBRC 113963 T ). In addition, the comparison of the whole genome sequences and phenotypic features suggested that Nonlabens tegetincola and Nonlabens sediminis belong to the same species. Therefore, it is proposed that N. sediminis is reclassified as a later heterotypic synonym of N. tegetincola .