Open AccessSpirosoma endbachense sp. nov., isolated from a natural salt meadow
Author(s) -
Julian Rojas,
Binoy Ambika Manirajan,
Stefan Ratering,
Christian Suarez,
Rita Geißler-Plaum,
Sylvia Schnell
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004601
Subject(s) - biology , 16s ribosomal rna , strain (injury) , genomic dna , oxidase test , bacteria , botany , bacteroidetes , gene , phylogenetic tree , biochemistry , genetics , enzyme , anatomy
A Gram-stain-negative bacterium, designated I-24 T , was isolated from soil of a natural salt meadow. Strain I-24 T was aerobic, non-motile, rod-shaped, catalase-positive, oxidase-positive and grew optimally at pH 7 and 25 °C. Comparative 16S rRNA gene analysis indicated that strain I-24 T has closest similarities to Spirosoma agri KCTC 52727 T (95.9 %) and Spirosoma terrae KCTC 52035 T (95.5 %). Strain I-24 T contained summed feature 3 (C 16 : 1 ω 7 c /C 16 : 1 ω 6 c ) and C 16 : 1 ω 5 c as the major fatty acids, the predominant respiratory quinone was menaquinone MK-7, and the major polar lipids were phosphatidylethanolamine as well as an unidentified phosphoaminolipid. The draft genome of strain I-24 T consists of 10 326 072 base pairs with 9153 predicted coding sequences and a G+C content of 47.7 mol%. Clear distinctions between strain I-24 T and S. agri KCTC 52727 T or S. terrae KCTC 52035 T were shown in the pairwise average nucleotide identity results with values of 76.71 and 74.01 %, respectively. Moreover, the digital DNA–DNA relatedness values to these strains were 20.8 and 19.0 %. Based on its phenotypic, genotypic and chemotaxonomic characteristics, strain I-24 T represents a novel species of the genus Spirosoma , for which the name Spirosoma endbachense sp. nov. is proposed. The type strain is I-24 T (DSM 111055 T =KCTC 72613 T ).