Open AccessMesobacillus harenae sp. nov., isolated from the sandy soil of a cold desert
Author(s) -
Gaosen Zhang,
Ruiqi Yang,
Tuo Chen,
Binglin Zhang,
Hui Yang,
Xiukun Wu,
Haining Gao,
Wei Zhang,
Guangxiu Lu
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004594
Subject(s) - 16s ribosomal rna , biology , strain (injury) , bacteria , diamino acid , phosphatidylglycerol , fatty acid , botany , microbiology and biotechnology , gene sequence , biochemistry , phospholipid , membrane , phosphatidylcholine , anatomy , genetics
A bacterial strain, designated Y40 T , was isolated from sandy soil sampled on the Qinghai–Tibet Plateau. A polyphasic study confirmed the affiliation of the strain with the genus Mesobacillus . Strain Y40 T was found to be an aerobic, Gram-stain-positive, motile and rod-shaped bacterium. The strain grew at 10–42 °C, pH 6–9 and with 0–2 % (w/v) NaCl. The diagnostic amino acid was meso -diaminopimeilic acid. MK7 was predominant menaquinone, and iso-C 15:0 , iso-C 17:1 ω10 c and anteiso-C 15:0 were the major fatty acids. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. The DNA G+C content was 40.6 mol%. Based on he results of 16S rRNA gene sequence analysis, strain Y40 T was phylogenetically closely related to Mesobacillus zeae JJ-247 T and Mesobacillus foraminis CV53 T , with similarities of 98.0 and 97.7 %, respectively. The average nucleotide identity (ANIb) values between strain Y40 T and Mesobacillus zeae JJ-247 T and Mesobacillus foraminis CV53 T were 69.9 and 70.0 %, respectively. Based on the morphological, physiological, and chemotaxonomic data, it is proposed that strain Y40 T (=CICC 24459 T =JCM 32794 T ) should be classified into the genus Mesobacillus as Mesobacillus harenae sp. nov.