Open AccessParaflavitalea devenefica sp. nov., isolated from urban soil
Author(s) -
Xiaoxiao Hou,
Hongliang Liu,
Yumang Shang,
Sidi Mao,
Shucheng Li,
Suying Feng,
Hongkuan Deng,
Limin Wang,
K. Charles Ling,
Chunyang Zhang,
Zhongfeng Ding,
Yan Gao,
Shuzhen Wei,
Zhiwei Chen
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004587
Subject(s) - 16s ribosomal rna , strain (injury) , biology , phylogenetic tree , genomic dna , bacteria , bacteroidetes , gene , microbiology and biotechnology , genetics , anatomy
A Gram-stain-negative, rod-shaped, mesophilic, milky white-pigmented, aerobic, non-spore-forming and non-flagellated bacterium, designated strain X16 T , was isolated from urban soil of Zibo, Shandong, China. According to 16S rRNA gene sequence analysis, the isolate showed highest similarities with Paraflavitalea soli 5GH32-13 T (97.6 %), Pseudoflavitalea soli KIS20-3 T (96.2 %), Pseudobacter ginsenosidimutans Gsoil 221 T (96.0 %) and Pseudoflavitalea rhizosphaerae T16R-265 T (95.8 %). The neighbour-joining tree based on 16S rRNA gene sequences showed that strain X16 T formed a subcluster with Paraflavitalea soli 5GH32-13 T , and the subcluster was closely related to Pseudoflavitalea soli KIS20-3 T , Pseudobacter ginsenosidimutans Gsoil 221 T and Pseudoflavitalea rhizosphaerae T16R-265 T . Strain X16 T also formed a subcluster with Paraflavitalea soli 5GH32-13 T in phylogenetic tree based on genomic sequences. The polar lipids are phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids, two unknown lipids and two unknown phospholipids. The major quinone of strain X16 T is menaquinone-7 and the main fatty acids (>10 % of total fatty acids) of strain X16 T are iso-C 15 : 0 , iso-C 17 : 0 3-OH and iso-C 15 : 1 G. The genome length of strain X16 T is 8.7 Mb with a DNA G+C content of 47.4 %. ANI values among strain X16 T and strain Paraflavitalea soli 5GH32-13 T , Pseudobacter ginsenosidimutans Gsoil 221 T , and Pseudoflavitalea rhizosphaerae T16R-265 T are 78.1, 70.7, 70.6 %, respectively. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain X16 T represents a novel species. Besides, strain X16 T can detoxify high toxicity selenite [Se(IV)] to low toxicity elemental selenium [Se(0)], for which the name Paraflavitale devenefica sp. nov. is proposed. The type strain is X16 T (=KACC 21698 T =GDMCC1.1757 T ).