Open AccessKineobactrum salinum sp. nov., isolated from marine sediment
Author(s) -
Minji Kim,
InTae Cha,
KiEun Lee,
Byoung-Hee Lee,
Soo-Je Park
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004586
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , microbiology and biotechnology , phosphatidylglycerol , genus , genomic dna , bacteria , gene , botany , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
Strain M2 T , isolated from marine sediment collected at Jeju Island, was an aerobic, Gram-stain-negative, oxidase- and catalase-positive, motile, rod-shaped bacterium that formed circular, raised, yellow colonies. Strain M2 T grew at 15–42 °C, pH 5.5–9.0 and with 1–9 % (w/v) NaCl. Phylogenetic analysis based on its 16S rRNA gene sequences indicated that strain M2 T was closely related to Kineobactrum sediminis F02 T (98.0 % sequence similarity). Ubiquinone-8 was determined to be the sole respiratory quinone. Summed feature 3 (C 16 : 1 ω 6 c /C 16 : 1 ω 7 c ) and summed feature 8 (C 18 : 1 ω 7 c /C 18 : 1 ω 6 c ) were identified as the predominant fatty acids. The DNA G+C content and digital DNA–DNA relatedness between strain M2 T and K. sediminis F02 T were 60.7 mol% and 19.5 %, respectively. Phosphatidylglycerol and phosphatidylethanolamine were identified as the major polar lipids. Thus, polyphasic characterization revealed that strain M2 T represents a novel species in the genus Kineobactrum , for which the name Kineobactrum salinum sp. nov. is proposed. The type strain is M2 T (=KCTC 72815 T =VTCC 910108 T ).