Oricola thermophila sp. nov., a marine bacterium isolated from tidal flat sediment and emended description of the genus Oricola Hameed et al. 2015

A Gram-stain-negative, facultatively anaerobic, rod-shaped (1.8–4.4×0.5–0.7 µm) and motile marine bacterium, designated as MEBiC13590 T , was isolated from tidal flat sediment sampled at Incheon City, on the west coast of the Republic of Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC13590 T showed high similarity to Oricola cellulosilytica CC-AMH-0 T (98.2 %), followed by Oceaniradius stylonematis StC1 T (97.5 %); however, it clustered with Oricola cellulosilytica . The phylogenomic tree inferred by the up-to-date bacterial core gene set suggested that strain MEBiC13590 T shared a phyletic line with Oricola cellulosilytica . Average nucleotide identity and digital DNA–DNA hybridization values (75.0 and 19.3 %, respectively) between strain MEBiC13590 T and Oricola cellulosilytica CC-AMH-0 T were below the respective species delineation cutoffs. Growth was observed at 22–50 °C (optimum, 45 °C), at pH 5–9 (optimum, pH 7) and with 1–6 % (optimum, 3 %) NaCl. The predominant cellular fatty acids were C 16 : 0 (7.6 %), C 18 : 0 (12.2 %), 11-methyl C 18 : 1  ω7 c (5.7 %), C 19 : 0 cyclo ω6 c and summed feature 8 (comprising C 18 : 1  ω7 c and/or C 18 : 1  ω6 c ; 38 %). The DNA G+C content was 63.5 mol%. The major respiratory quinone was Q-10. Several phenotypic characteristics such as growth temperature, oxygen requirement, enzyme activities of urease, gelatinase, lipase (C 14 ), α-chymotrypsin, acid phosphatase, β-galactosidase, β-glucosidase etc. differentiate strain MEBiC13590 T from Oricola cellulosilytica CC-AMH-0 T . Based on this polyphasic taxonomic data, strain MEBiC13590 T should be classified as representing a novel species in the genus Oricola for which the name Oricola thermophila sp. nov. is proposed . The type strain is MEBiC13590 T (=KCCM 43313 T =JCM 33661 T ).