Acinetobacter lanii sp. nov., Acinetobacter shaoyimingii sp. nov. and Acinetobacter wanghuae sp. nov., isolated from faeces of Equus kiang

Six aerobic, non-motile, non-haemolytic, Gram-stain-negative, oxidase-negative strains (185 T , 187, 323-1 T , 194, dk386 T and dk771) were recovered from different faecal samples of Equus kiang on the Qinghai–Tibet Plateau. In the 16S rRNA gene sequences, one strain pair, 185 T /187, shared highest similarity to Acinetobacter equi 114 T (97.9 %), and the other two (323-1 T /194 and dk771 T /dk386) to Acinetobacter harbinensis CGMCC 1.12528 T (98.6 and 97.0 %, respectively). Phylogenomic tree analysis showed that these six strains formed three separate clades in the genus Acinetobacter . Digital DNA–DNA hybridization values of each pair of the isolates with all members of the genus Acinetobacter were far below 70 %. The main cellular fatty acids of all six strains were C 18 : 1  ω 9 c , C 16 : 0 and summed feature 3 (C 16 : 1  ω 7 c /C 16 : 1  ω 6 c ). Q-9 was the predominant respiratory quinone for strains 185 T , 323-1 T and dk386 T . The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the genotypic, phenotypic and biochemical analyses, these six strains represent three novel species of the genus Acinetobacter , for which the names Acinetobacter lanii sp. nov., Acinetobacter shaoyimingii sp. nov. and Acinetobacter wanghuae sp. nov. are proposed. The type strains are 185 T (=CGMCC 1.13636 T =JCM 33607 T ), 323-1 T (=CGMCC 1.13940 T =JCM 33608 T ) and dk386 T (=CGMCC 1.16589 T =JCM 33592 T ), respectively.