Open AccessLentilactobacillus kribbianus sp. nov., isolated from the small intestine of a mini pig
Author(s) -
Lu Bai,
Jayoung Paek,
Yeseul Shin,
Ho-Yong Park,
Young-Hyo Chang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004560
Subject(s) - biology , 16s ribosomal rna , housekeeping gene , phylogenetic tree , strain (injury) , genomic dna , bacteria , gene , sequence analysis , phylogenetics , microbiology and biotechnology , genetics , anatomy , gene expression
A Gram-stain-positive, facultative anaerobic, rod-shaped bacteria isolated from the small intestine of a mini pig was designated as strain YH-lac9 T . 16S rRNA gene sequence analysis revealed that the strain belongs to the genus Lentilactobacillus and is closely related to Lentilactobacillus senioris JCM 17472 T , Lentilactobacillus rapi JCM 15042 T and Lentilactobacillus diolivorans JCM 13927 T , with 97.6, 96.2 and 95.7 % sequence similarity, respectively. Analysis of housekeeping gene sequences ( pheS and recA ) revealed that the strain formed a sub-cluster with L. senioris , supporting the results of 16S rRNA gene sequences analysis. The average nucleotide identity value for YH-lac9 T and the most closely related strain is 74.1 %. The main fatty acids are C 18 : 1 ω9 c , summed feature 7, C 16 : 0 and summed feature 8. The G+C content of the genomic DNA is 37.8 mol%. In view of its chemotaxonomic, phenotypic and phylogenetic properties, YH-lac9 T (=KCTC 25005=JCM 33997) represents a novel taxon. The name Lentilactobacillus kribbianus sp. nov. is proposed.