Open AccessOrnithinimicrobium pratense sp. nov., isolated from meadow soil
Author(s) -
Qiannan Guo,
Bo Wang,
Zhengfu Zhou,
Ke Xia,
Lijuan Zhang,
Ming Chen,
Min Lin,
Wei Wang,
Wei Zhang,
Xufeng Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004557
Subject(s) - biology , 16s ribosomal rna , strain (injury) , diamino acid , genome size , glycolipid , peptidoglycan , phylogenetic tree , genomic dna , microbiology and biotechnology , bacteria , genome , gene , botany , cell wall , gene sequence , genetics , biochemistry , anatomy
A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204 T , was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204 T was closely related to Ornithinimicrobium flavum CPCC 203535 T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582 T (96.9 %), Serinicoccus sediminis GP-T3-3 T (96.8 %), Serinicoccus hydrothermalis JLT9 T (96.7 %), Ornithinimicrobium cerasi CPCC 203383 T (96.6 %) and Ornithinimicrobium kibberense K22-20 T (96.6 %). However, the digital DNA–DNA genome hybridization value between strain W204 T and the closest related strain O. flavum CPCC 203535 T was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H 4 ). The predominant cellular fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 and C 16 : 0 . The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204 T should be classified as representative of a novel species of the genus Ornithinimicrobium , for which the name Ornithinimicrobium pratense sp. nov. is proposed. The type strain is W204 T (=GDMCC 1.1391 T =KCTC 49237 T ).