Open AccessMicromonospora fluminis sp. nov., isolated from mountain river sediment
Author(s) -
Miladis Camacho-Pozo,
Anneleen D. Wieme,
Suyén Rodríguez Pérez,
Gabriel Llauradó,
Charlotte Peeters,
Cindy Snauwaert,
Yaneisy Lescaylle Veranes,
Leonor Peña Zamora,
Peter Schümann,
Peter Vandamme
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004554
Subject(s) - micromonospora , biology , 16s ribosomal rna , microbiology and biotechnology , mycelium , strain (injury) , spore , bacteria , botany , genetics , streptomyces , anatomy
During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38 T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38 T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso -diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC 15:0 , 10 methyl C 17:0 , anteiso-C 17:0 and iso-C 17:0 . The predominant menaquinones were MK-10(H 4 ) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142 T (99.5 %), Micromonospora citrea DSM 43903 T (99.4 %), Micromonospora marina DSM 45555 T (99.4 %), Micromonospora maritima DSM 45782 T (99.3 %), Micromonospora sediminicola DSM 45794 T (99.3 %), Micromonospora aurantiaca DSM 43813 T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246 T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026 T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38 T as a novel species. On the basis of the results presented here, we propose to classify strain A38 T (=LMG 30467 T =CECT 30034 T ) as the type strain of the novel species Micromonospora fluminis sp. nov.