Open AccessPseudopontixanthobacter vadosimaris gen. nov., sp. nov., isolated from shallow sea near Kueishan Island
Author(s) -
Jia Sun,
Jiaying Guo,
Ta-Hui Lin,
Xuejin Feng,
Rui Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004552
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , phosphatidylglycerol , strain (injury) , glycolipid , pigment , fatty acid , phospholipid , phosphatidylethanolamine , oxidase test , botany , gene , biochemistry , phosphatidylcholine , microbiology and biotechnology , chemistry , enzyme , anatomy , organic chemistry , membrane
A Gram-stain-negative and aerobic bacterial strain, designated as JL3514 T , was isolated from surface water of the hydrothermal system around Kueishan Island. The isolate formed red colonies and cells were non-flagellated, rod-shaped and contained methanol-soluble pigments. Growth was observed at 10–50 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and in the presence of 0–9 % (w/v) NaCl (optimum, 2 %). Strain JL3514 T was positive for catalase and weakly positive for oxidase. Results of 16S rRNA gene sequence analyses showed highest similarities to species in the family Erythrobacteraceae , namely Croceibacterium atlanticum (96.1 %), Pelagerythrobacter marensis (96.0 %), Tsuneonella rigui (96.0 %) and Altericroceibacterium xinjiangense (96.0 %). Phylogenetic analysis based on core gene sequences revealed that the isolate formed a distinct branch with the related species and it had a lower average amino acid identity value than the suggested threshold for genera boundaries. The major fatty acids (>5 %) were summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ), summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ), C 16 : 0 , C 17 : 1 ω 6 c , C 14 : 0 2-OH and C 12 : 0 . The dominant polar lipids comprised diphosphatidylglycerol, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, glycolipid, two unidentified lipids and one unidentified phospholipid. The main respiratory quinones were ubiquinone-10 (95.7 %) and ubiquinone-9 (4.3 %). The DNA G+C content from the genome was 63.0 mol%. Based on the presented data, we consider strain JL3514 T to represent a novel genus of the family Erythrobacteraceae , with the name Pseudopontixanthobacter vadosimaris gen. nov., sp. nov. The type strain is JL3514 T (=KCTC 62623 T =MCCC 1K03561 T ).