Open AccessAquipluma nitroreducens gen. nov. sp. nov., a novel facultatively anaerobic bacterium isolated from a freshwater lake
Author(s) -
Miho Watanabe,
Hisaya Kojima,
Manabu Fukui
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004551
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , bacteria , bacteroidetes , phylogenetics , nitrite , microbiology and biotechnology , botany , gene , nitrate , ecology , genetics , anatomy
A novel facultatively anaerobic, nitrate-reducing bacterium, designated MeG22 T , was isolated from a freshwater lake in Japan. Cells of the strain were straight rods (0.8×2.5–10 µm), motile, and Gram-stain-negative. For growth, the optimum NaCl concentration was 0 % and the optimum temperature was 30 °C. Under anoxic conditions, strain MeG22 T reduced nitrate to nitrite. Major cellular fatty acids were C 15 : 1 ω6 c (13.6 %), C 17 : 0 (11.9 %), anteiso-C 15 : 0 (10.6 %) and iso-C 15 : 0 (10.6 %). The major respiratory quinone was menaquinone-7. The genome sequence of strain MeG22 T consists of 5 712 279 bp with a G+C content of 40.3 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family Prolixibacteraceae within the phylum Bacteroidetes . The closest relative of strain MeG22 T was Sunxiuqinia faeciviva strain JAM-BA0302 T with a 16S rRNA gene sequence similarity of 90.9 %. On the basis of phylogenetic and phenotypic characterization, Aquipluma nitroreducens , gen. nov., sp. nov., belonging to the family Prolixibacteraceae is proposed with the type strain MeG22 T (=NBRC 112896 T =DSM 106262 T ).