Open AccessNocardioides convexus sp. nov. and Nocardioides anomalus sp. nov., isolated from soil and mineral water
Author(s) -
Jinah Park,
Qingzhen Liu,
Soohyun Maeng,
Won Jung Choi,
Yoonjee Chang,
WanTaek Im
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004547
Subject(s) - biology , microbiology and biotechnology , 16s ribosomal rna , genetics , bacteria
Two bacterial strains designated as W3-2-3 T and HKS04 T were isolated from mineral water and a soil sample, respectively, in the Republic of Korea. The 16S rRNA genes of the two strains shared a sequence similarity of 93.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains W3-2-3 T and HKS04 T formed a distinct lineage within the genus Nocardioides of the family Nocardioidaceae (order Propionibacteriales ). The closely related species of strain W3-2-3 T were Nocardioides albidus (98.9 %), Nocardioides caeni (98.8 %), Nocardioides kongjuensis (98.6 %), Nocardioides aromaticivorans (98.5 %), Nocardioides nitrophenolicus (98.4 %), Nocardioides flava (98.2 %) and Nocardioides ginsengisoli (98.1 %). The closest species of strain HKS04 T was Nocardioides halotolerans (98.7 %). The genome sizes of strains W3-2-3 T and HKS04 T were 4741198 and 5 120341 bp, respectively. The genomic DNA G+C contents of strains W3-2-3 T and HKS04 T were 73.3 and 72.1 mol%, respectively. The main fatty acids of strain W3-2-3 T were C 17:1 ω 6 c and iso-C 16:0 and those of strain HKS04 T were iso-C 16:0 and iso-C 16:0 H. The main polar lipids of both strains were diphosphatidylglycerol and phosphatidylglycerol and the predominant respiratory quinone was MK-8(H 4 ), supporting the affiliation of these strains with the genus Nocardioides . Based on the results of biochemical, chemotaxonomic and phylogenetic analyses, two novel species, Nocardioides convexus W3-2-3 T (KACC 21211 T =LMG 31251 T ) and Nocardioides anomalus HKS04 T (KACC 18879 T =LMG 31249 T ), are proposed.