Open AccessAlteromonas ponticola sp. nov., a gammaproteobacterium isolated from seawater
Author(s) -
YoungOk Kim,
In-Suk Park,
Sooyeon Park,
JungHoon Yoon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004545
Subject(s) - alteromonas , biology , 16s ribosomal rna , phylogenetic tree , genus , phylogenetics , botany , type species , strain (injury) , microbiology and biotechnology , bacteria , gene , genetics , anatomy
A Gram-stain-negative, aerobic, non-spore-forming, non-motile and ovoid or rod-shaped bacterial strain, MYP5 T , was isolated from seawater in Jeju island of South Korea. MYP5 T grew optimally at 30–35 °C and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that MYP5 T fell within the clade enclosed by the type strains of species of the genus Alteromonas , clustering with the type strains of Alteromonas confluentis and Alteromonas halophila . MYP5 T exhibited the highest 16S rRNA gene sequence similarity value (98.0 %) to the type strain of A. confluentis and similarities of 95.1–97.9 % to the type strains of the other species of the genus Alteromonas . ANI and dDDH values of genomic sequences between MYP5 T and the type strains of 22 species of the genus Alteromonas were 66.8–70.5 % and 18.6–27.5 %, respectively. The DNA G+C content of MYP5 T , determined from the genome sequence, was 46.1 %. MYP5 T contained Q-8 as the predominant ubiquinone and C 18 : 1 ω7 c , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), C 16 : 0 and 10-methyl C 17 : 0 as the major fatty acids. The major polar lipids of MYP5 T were phosphatidylethanolamine and phosphatidylglycerol. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that MYP5 T is separated from species of the genus Alteromonas . On the basis of the data presented, MYP5 T is considered to represent a novel species of the genus Alteromonas , for which the name Alteromonas ponticola sp. nov. is proposed. The type strain is MYP5 T (=KCTC 82144 T =NBRC 114354 T ).