Open AccessNoviherbaspirillum aerium sp. nov., isolated from air
Author(s) -
Xue Han,
Chungen Piao,
Yongjun Lin,
Dan-Ran Bian,
Yong Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004537
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , phosphatidylglycerol , strain (injury) , botany , microbiology and biotechnology , bacteria , phospholipid , gene , biochemistry , genetics , anatomy , phosphatidylcholine , membrane
A Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped, motile with polar flagella and pale-orange bacterium, designated strain 122213-3 T , was isolated from air, collected at the foot of the Xiangshan Mountain, located in Beijing, PR China. Optimal growth occurred at 28 °C, at pH 7 and in the presence of 0–1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that 122213-3 T clustered with species of the genus Noviherbaspirillum and formed a distinct sublineage, showing highest similarities to Noviherbaspirillum malthae CC-AFH3 T (96.88 %), Noviherbaspirillum massiliense JC206 T (95.78 %) and Noviherbaspirillum aurantiacum SUEMI08 T (95.78 %). The predominant cellular fatty acids were summed feature 3 (C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c ), summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) and C 16 : 0 . The predominant quinone was ubiquinone 8 (Q-8). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid and two unidentified polar lipids. The polyamine pattern showed the presence of putrescine as the major polyamine, with minor amounts of 2-hydroxyputrescine. The DNA G+C content was 60.1 mol%. The phylogenetic analysis and physiological and biochemical data showed that strain 122213-3 T should be classified as representing a novel species in the genus Noviherbaspirillum , for which the name Noviherbaspirillum aerium sp. nov. is proposed. The type strain of N. aerium is 122213-3 T (=CFCC 14286 T =LMG 30131 T ).