Noviherbaspirillum aerium sp. nov., isolated from air

A Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped, motile with polar flagella and pale-orange bacterium, designated strain 122213-3 T , was isolated from air, collected at the foot of the Xiangshan Mountain, located in Beijing, PR China. Optimal growth occurred at 28 °C, at pH 7 and in the presence of 0–1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that 122213-3 T clustered with species of the genus Noviherbaspirillum and formed a distinct sublineage, showing highest similarities to Noviherbaspirillum malthae CC-AFH3 T (96.88 %), Noviherbaspirillum massiliense JC206 T (95.78 %) and Noviherbaspirillum aurantiacum SUEMI08 T (95.78 %). The predominant cellular fatty acids were summed feature 3 (C 16 : 1  ω 6 c and/or C 16 : 1  ω 7 c ), summed feature 8 (C 18 : 1  ω 7 c and/or C 18 : 1  ω 6 c ) and C 16 : 0 . The predominant quinone was ubiquinone 8 (Q-8). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid and two unidentified polar lipids. The polyamine pattern showed the presence of putrescine as the major polyamine, with minor amounts of 2-hydroxyputrescine. The DNA G+C content was 60.1 mol%. The phylogenetic analysis and physiological and biochemical data showed that strain 122213-3 T should be classified as representing a novel species in the genus Noviherbaspirillum , for which the name Noviherbaspirillum aerium sp. nov. is proposed. The type strain of N. aerium is 122213-3 T (=CFCC 14286 T =LMG 30131 T ).