Open AccessRadiobacillus deserti gen. nov., sp. nov., a UV-resistant bacterium isolated from desert soil
Author(s) -
Juan Li,
Binglin Zhang,
Guangxiu Lu,
Yang Liu,
Hui Yang,
Ruiqi Yang,
Yuqin Huang,
Shi-Weng Li,
Tuo Chen,
Wei Zhang,
Gaosen Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004536
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , phylogenetic tree , diamino acid , halophile , bacteria , microbiology and biotechnology , botany , diaminopimelic acid , gene , cell wall , gene sequence , biochemistry , genetics , anatomy
A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69 T , was isolated from sandy soil sampled in the Taklimakan Desert. The strain grew at 20–50 °C, pH 6–9 and with 0–12 % (w/v) NaCl. The major fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and C 16 : 0 . The only respiratory quinone was MK-7. The cell-wall peptidoglycan was meso -diaminopimelic acid. Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids. Genomic DNA analysis revealed a G+C content of 38.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69 T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331 T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae . Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69 T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp. nov. is proposed, with the type strain being Radiobacillus deserti TKL69 T (=JCM 33497 T =CICC 24779 T ).