Open AccessArenibacterium halophilum gen. nov., sp. nov., a halotolerant bacterium in the family Rhodobacteraceae isolated from a coastal sand dune
Author(s) -
Jihye Baek,
Jong-Hwa Kim,
JungHoon Yoon,
Jung-Sook Lee,
Ampaitip Sukhoom,
Wonyong Kim
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004534
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , ribosomal rna , strain (injury) , microbiology and biotechnology , halotolerance , whole genome sequencing , genomic dna , bacteria , gene , genetics , genome , anatomy
A Gram-stain-negative, non-pigmented, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1492 T , was isolated from a coastal sand dune and its taxonomic position was examined using a polyphasic approach. Cells of strain CAU 1492 T grew optimally at 30 °C, pH 7.0 and in 3 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1492 T showed that it formed a distinct lineage within the family Rhodobacteraceae as a separate deep branch, with 96.8 % or lower sequence similarity values to representatives of the genera Marivita , Donghicola , Sulfitobacter , Marinovum , Phaeobacter , Primorskyibacter , Roseovarius and Aestuariihabitans . Strain CAU 1492 T was closely related to Marivita geojedonensis DPG-138 T (96.8 %), Donghicola eburneus SW-277 T (96.7 %), Sulfitobacter porphyrae SCM-1 T (96.7 %), Marinovum algicola FF3 T (96.6 %) and Aestuariihabitans beolgyonensis BB-MW15 T (96.4 %) based on 16S rRNA gene sequences. The major cellular fatty acids of strain CAU 1492 T were cyclo-C 19 : 0 ω 8 c and summed feature 8 (C 18 : 1 ω 7 c /C 18 : 1 ω 6 c ). The polar lipid pattern was composed of phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified aminolipid. The strain contained Q-10 as the sole respiratory quinone. The draft genome of strain CAU 1492 T was 4.63 Mb with a DNA G+C content of 63.1 mol%. The genome includes 4292 protein-coding genes and a five rRNA operons. On the basis of the phenotypic, chemotaxonomic and genomic data, strain CAU 1492 T represents a novel genus in the family Rhodobacteraceae for which the name Arenibacterium halophilum gen. nov., sp. nov. is proposed. The type strain of Arenibacterium halophilum is CAU 1492 T (=KCTC 62998 T =NBRC 113696 T ).