Open AccessPseudoalteromonas caenipelagi sp. nov., isolated from a tidal flat
Author(s) -
Sooyeon Park,
Seo Yeon Lee,
Wonyong Kim,
JungHoon Yoon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004532
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , clade , flagellum , bacteria , microbiology and biotechnology , botany , gene , genetics , anatomy
A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated JBTF-M23 T , was isolated from tidal flat sediment collected from the Yellow Sea, Republic of Korea. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M23 T fell within the clade comprising the type strains of Pseudoalteromonas species, clustering with the type strains of P. byunsanensis and P. amylolytica . Strain JBTF-M23 T exhibited the highest 16S rRNA gene sequence similarity value (98.6 %) to the type strain of P. rubra and sequence similarities of 98.3 and 97.7 % to the type strains of P. byunsanensis and P. amylolytica, respectively. The DNA G+C content of strain JBTF-M23 T from genomic sequence data was 41.98 %. The ANI and dDDH values between strain JBTF-M23 T and the type strains of P. rubra , P. byunsanensis and P. amylolytica were 71.3–76.6 and 19.4–19.9 %, respectively. Strain JBTF-M23 T contained Q-8 as the predominant ubiquinone and C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c , C 16 : 0 and C 18 : 1 ω 7 c as the major fatty acids. The major polar lipids of strain JBTF-M23 T were phosphatidylethanolamine and one unidentified aminolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M23 T is separated from recognized Pseudoalteromonas species. On the basis of the data presented, strain JBTF-M23 T is considered to represent a novel species of the genus Pseudoalteromonas , for which the name Pseudoalteromonas caenipelagi sp. nov. is proposed. The type strain is JBTF-M23 T (=KACC 19900 T =NBRC 113647 T ).