Open AccessMarinobacter halodurans sp. nov., a halophilic bacterium isolated from sediment of a salt flat
Author(s) -
Yeonjae Yoo,
Hanbyul Lee,
Bong-Oh Kwon,
Jong Seong Khim,
Seung-Woo Baek,
Duleepa Pathiraja,
Byeonghyeok Park,
In Geol Choi,
Gyu Hyeok Kim,
Beom Seok Kim,
Jae Jin Kim
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004530
Subject(s) - biology , 16s ribosomal rna , bacteria , halophile , microbiology and biotechnology , strain (injury) , anatomy , genetics
A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2 T , was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2 T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18 % (w/v) NaCl (optimum 6 %). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2 T was closely related to Marinobacter segnicrescens SS011B1-4 T (97.0 %) followed by, ' Marinobacter nanhaiticus ' D15-8W (96.7 %), Marinobacter bryozoorum 50-11 T (96.7 %), Marinobacter koreensis DSMZ 179240 T T (96.5 %) and Marinobacter bohaiensis T17 T (96.5 %). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2 T and related type strains were 73.7–79.8 and 19.9–22.5 %, and also 73.5 and 20.7 % with Marinobacter hydrocarbonoclasticus . YJ-S3-2 T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C 16 : 0 (22.3 %), summed feature 9 (C 17 : 1 iso ω9 c /C 16 : 0 10-methyl, 13.8 %) and 3 (C 16 : 1 ω7 c/ C1 6 : 1 ω6 c, 11.9 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2 T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2 T should be classified as representing a novel species within the genus Marinobacter , for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2 T (=KACC 19883 T =KCTC 62937 T =JCM 33109 T ).