Open AccessAdhaeribacter arboris sp. nov., and Adhaeribacter pallidiroseus sp. nov., novel bacteria isolated from birch tree (Betula platyphylla)
Author(s) -
Heeyoung Kang,
Haneul Kim,
Inseong Cha,
Kiseong Joh
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004516
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , bacteria , microbiology and biotechnology , botany , phylogenetics , betula platyphylla , gene , genetics
Two Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strains, designated as HMF7605 T and HMF7616 T , were isolated from birch tree, in Yong-in, Republic of Korea. Strains HMF7605 T and HMF7616 T exhibited the highest 16S rRNA gene sequence similarities of 95.9 and 97.5 % to Adhaeribacter swui 17mud1-7 T , 97.9 % between themselves. The values of average nucleotide identity and in silico DNA–DNA hybridization between strains HMF7605 T and HMF7616 T were 77.6 and 22.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains revealed that they belonged to the genus Adhaeribacter within the family Hymenobacteraceae . The predominant fatty acids of both strains were iso-C 15 : 0 , summed feature 3 (comprising C 16 : 1 ω7 c and/or C 16 : 1 ω 6c), C 16 : 1 ω 5c and summed feature 4 (comprising iso-C 17 : 1 I and/or anteiso-C 17 : 1 B). The both strains contained menaquinone-7 as the only isoprenoid quinone. The major polar lipid profiles of the two strains were similar with phosphatidylethanolamine, one unidentified aminophosphoglycolipid, one unidentified glycolipid and three unidentified polar lipids. The DNA G+C contents of strains HMF7605 T and HMF7616 T were 42.0 and 42.8 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, Adhaeribacter arboris sp. nov. and Adhaeribacter pallidiroseus sp. nov. are proposed. The type strains are HMF7605 T (=KCTC 62465 T =NBRC 113228 T ) and HMF7616 T (=KCTC 62466 T =NBRC 113229 T ), respectively.