Open AccessRheinheimera mangrovi sp. nov., a bacterium isolated from mangrove sediment
Author(s) -
Weiyan Zhang,
Suting Zhu,
YuPing Cheng,
Lijian Ding,
Shengying Li,
Nan Peng,
Shan He
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004513
Subject(s) - biology , 16s ribosomal rna , strain (injury) , bacteria , oxidase test , phosphatidylglycerol , microbiology and biotechnology , phospholipid , biochemistry , genetics , enzyme , anatomy , membrane , phosphatidylcholine
A Gram-stain-negative, rod-shaped, motile and strictly aerobic bacterium, designated LHK132 T , was isolated from a mangrove sediment sample collected in Haikou, Hainan Province, PR China. Strain LHK132 T was able to grow at temperatures of 10–45 °C, at salinities of 0–7.0 % (w/v) and at pH 6.0–9.0. Catalase and oxidase activities, H 2 S production, urease and methyl red reaction were positive. Indole, nitrate reduction, hydrolysis of gelatin, starch, casein and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acids were C 16 : 0 and summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. According to 16S rRNA gene sequence analysis, strain LHK132 T had 98.3, 97.5, 97.4, 97.2 and 97.1% similarities to Rheinheimera soli BD-d46 T , Rheinheimera sediminis YQF-1 T , Rheinheimera tangshanensis JA3-B52 T , Rheinheimera mesophila IITR-13 T and Rheinheimera arenilitoris J-MS1 T , respectively. Phylogenetic analyses indicated that strain LHK132 T formed a distinct lineage with R. soli BD-d46 T within the genus Rheinheimera . The average nucleotide identity and digital DNA–DNA hybridization values between strain LHK132 T and related species of the genus Rheinheimera were well below the thresholds for species delineation. The DNA G+C content was 46.7 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic data, strain LHK132 T is considered a representative of a novel species in the genus Rheinheimera , for which the name Rheinheimera mangrovi sp. nov. is proposed. The type strain is LHK132 T (=KCTC 62580 T =MCCC 1K03529 T ).