Open AccessNovel acetic acid bacteria from cider fermentations: Acetobacter conturbans sp. nov. and Acetobacter fallax sp. nov
Author(s) -
Atena Sadat Sombolestani,
Ilse Cleenwerck,
Margo Cnockaert,
Wim Borremans,
Anneleen D. Wieme,
Luc De Vuyst,
Peter Vandamme
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004511
Subject(s) - acetobacter , biology , microbiology and biotechnology , acetic acid bacteria , bacteria , strain (injury) , genetics , anatomy
Strains LMG 1627 T , LMG 1636 T and LMG 1637 were all isolated from cider fermentations in the 1940s and 1950s. A recent study based on MALDI-TOF MS and dnaK gene sequence analyses suggested they represented novel Acetobacter species. In the present study, we determined the whole-genome sequences of these strains and analysed their phenotypic and chemotaxonomic characteristics. A phylogenomic analysis based on 107 single-copy core genes revealed that they represented a single Acetobacter lineage with Acetobacter aceti , Acetobacter sicerae , Acetobacter musti and Acetobacter oeni , Acetobacter estunensis and with Acetobacter nitrogenifigens as an outgroup to this cluster. OrthoANIu value and dDDH analyses among these and other Acetobacter type strains confirmed that these three strains represented two novel Acetobacter species, which could be differentiated from other closely related type strains of Acetobacter by different phenotypic tests, such as ketogenesis from glycerol. We therefore propose to classify strain LMG 1627 T in the novel species Acetobacter conturbans sp. nov., with LMG 1627 T (=NCIMB 8945 T ) as the type strain, and to classify strains LMG 1636 T and LMG 1637 in the novel species Acetobacter fallax sp. nov., with LMG 1636 T (=NCIMB 8956 T ) as the type strain.