Open AccessTabrizicola algicola sp. nov. isolated from culture of microalga Ettlia sp.
Author(s) -
Chan Yeong Park,
Seong-Jun Chun,
Chun-Zhi Jin,
Ve Van Le,
Yingshun Cui,
Song Yeon Kim,
ChiYong Ahn,
HeeMock Oh
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004508
Subject(s) - biology , 16s ribosomal rna , strain (injury) , genomic dna , phylogenetic tree , genome size , microbiology and biotechnology , ribosomal rna , genome , bacteria , botany , gene , genetics , anatomy
A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile, and rod-shaped bacterium, strain ETT8 T was isolated from a chemostat culture of microalga Ettlia sp. YC001. Optimal growth was with 0–2% NaCl and at 25–37 °C on R2A medium. Phylogenetic analysis based on the 16S rRNA gene and genome sequence showed that strain ETT8 T belongs to the genus Tabrizicola , with the close neighbours being T. sediminis DRYC-M-16 T (98.1 %), T. alkalilacus DJC T (97.6 %), T. fusiformis SY72 T (96.9 %), T. piscis K13M18 T (96.8 %), and T. aquatica RCRI19 T (96.5 %). The genomic comparison of strain ETT8 T with type species in the genus Tabrizicola was analysed using the genome-to-genome distance calculator (GGDC), average nucleotide identity (ANI), and average amino acid identity (AAI) (values indicated ≤17.7, ≤75.4 and ≤71.9 %, respectively). The genomic DNA G+C content of strain ETT8 T was 64.4 %, plus C 18 : 1 ω6 c and C 18 : 0 -iso were the major fatty acids and Q-10 the major respiratory quinone. Strain ETT8 T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine aminolipid, and four unidentified lipids as the major polar lipids. Based on the chemotaxonomic, genotypic, and phenotype results, strain ETT8 T was recognized as a novel species of the genus Tabrizicola for which the name Tabrizicola algicola sp. nov. is proposed. The type strain is ETT8 T (=KCTC 72206 T =JCM 31893 T =MCC 4339 T ).