Open AccessSphingobacterium chungjuense sp. nov., isolated from a freshwater lake
Author(s) -
Jaeho Song,
Yochan Joung,
Shan-Hui Li,
Juchan Hwang,
Jang-Cheon Cho
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004507
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , whole genome sequencing , genome size , genome , bacteria , genus , microbiology and biotechnology , gene , genetics , botany , anatomy
A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated strain IMCC25678 T , was isolated from an artificial freshwater reservoir, Chungju Lake, in the Republic of Korea. The 16S rRNA gene sequence analysis indicated that strain IMCC25678 T belongs to the genus Sphingobacterium with ≤98.7 % sequence similarities to Sphingobacterium species. Whole genome sequencing of strain IMCC25678 T revealed a 3.9 Mbp genome size with a DNA G+C content of 42.2 mol%. The IMCC25678 T genome shared ≤89.7 % average nucleotide identity and ≤21.4 % digital DNA–DNA hybridization values with closely related species of the genus Sphingobacterium , indicating that the strain represents a novel species. Summed feature 3 (C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c ), iso-C 15 : 0 and iso-C 17 : 0 3-OH were found to be the predominant cellular fatty acid constituents in the strain. The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unidentified phosphoglycolipid, one unidentified sphingolipid and three unidentified polar lipids. Based on the phylogenetic and phenotypic characteristics, strain IMCC25678 T was considered to represent a novel species within the genus Sphingobacterium , for which the name Sphingobacterium chungjuense sp. nov. is proposed. The type strain is IMCC25678 T (=KACC 19485 T =NBRC 113130 T ).