Open AccessBifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp. nov.
Author(s) -
Mónica Modesto,
Maria Satti,
Koichi Watanabe,
ChienHsun Huang,
JongShian Liou,
Tomohiko Tamura,
Satomi Saito,
Koji Mori,
Lina Huang,
Camillo Sandri,
Caterina Spiezio,
Barbara Sgorbati,
Donatella Scarafile,
Cesare Cammà,
Massimo Ancora,
Claudio Patavino,
Masanori Arita,
Paola Mattarelli
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004506
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , ribosomal rna , strain (injury) , housekeeping gene , genetics , microbiology and biotechnology , gene , anatomy , gene expression
Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth ( Choloepus didactylus ) housed in Parco Natura Viva, in Italy. Comparative sequence analysis of 16S rRNA and of five housekeeping ( hsp 60, rpo B, clp C, dna J, dna G) genes revealed that these strains were classified into two clusters. On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp. kashiwanohense DSM 21854 T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6 T ), whereas the type strain of Bifidobacterium dentium DSM 20436 T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II. The average nucleotide identity (ANI) values of BRDM 6 T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively. Therefore, genotyping based on the genome sequence of the strain BRDM 6 T combined with phenotypic analyses clearly revealed that the strain BRDM 6 T represents a novel species for which the names Bifidobacterium choloepi sp. nov. (BRDM 6 T =NBRC 114053 T =BCRC 81222 T ) is proposed.