Open AccessPseudarthrobacter psychrotolerans sp. nov., a cold-adapted bacterium isolated from Antarctic soil
Author(s) -
Yoonjae Shin,
Byoung Hee Lee,
Ki Eun Lee,
Woojun Park
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004505
Subject(s) - biology , 16s ribosomal rna , phosphatidylglycerol , bacteria , strain (injury) , psychrophile , phylogenetic tree , microbiology and biotechnology , glycolipid , ribosomal rna , fatty acid , gene , biochemistry , phospholipid , genetics , phosphatidylcholine , anatomy , membrane
A novel cold-tolerant bacterium, designated strain YJ56 T , was isolated from Antarctic soil collected from the Cape Burk area. Phylogenetic analysis through 16S rRNA gene sequence similarity revealed that strain YJ56 T was most closely related to the genus Pseudarthrobacter , including Pseudarthrobacter oxydans DSM 20119 T (99.06 % similarity), Pseudarthrobacter polychromogenes DSM 20136 T (98.98 %) and Pseudarthrobacter sulfonivorans ALL T (98.76 %). The genome size (5.2 Mbp) of strain YJ56 T was the largest among all the published genomes of Pseudarthrobacter type strains (4.2-5.0 Mbp). The genomic G+C content of strain YJ56 T (64.7 mol%) was found to be consistent with those of other Pseudarthrobacter strains (62.0-71.0 mol%). The average nucleotide identity and average amino acid identity values between strain YJ56 T and P. sulfonivorans ALL T were estimated at 84.1 and 84.2 %, respectively. The digital DNA-DNA hybridization value between the two strains was calculated to be 28.0 %. This rod-shaped and obligate aerobic strain exhibited no swimming or swarming motility. It had catalase activity but no oxidase activity. Cells grew at 4-28 °C (optimum, 13 °C) and pH 5.0-11.0 (optimum, pH 7.0) and with 0-6.0 % (w/v) NaCl (optimum, 0%) in Reasoner's 2A medium. MK-9 (H2) was the sole menaquinone. Two-dimensional TLC results revealed that the primary polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two glycolipids and phosphatidylinositol. Fatty acid methyl ester analysis showed that anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , C 16 : 0 and iso-C 16 : 0 were the major cellular fatty acids in strain YJ56 T . Based on phenotypic and genotypic characteristics, strain YJ56 T represents a novel species of the genus Pseudarthrobacter , and thus the name Pseudarthrobacter psychrotolerans sp. nov is proposed. The type strain is YJ56 T (=JCM 33881 T =KACC 21510 T ).