Open AccessRufibacter hautae sp. nov., a red-pigmented bacterium from freshwater lake sediment, and proposal of Rufibacter quisquiliarum as a latter heterotypic synonym of Rufibacter ruber
Author(s) -
Jia Zhou,
Wenwen Ma,
Jianhang Qu,
Haifeng Li,
Bang-Bang Yang,
Lingbo Qu,
Linfeng Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004502
Subject(s) - 16s ribosomal rna , biology , rpob , housekeeping gene , phylogenetic tree , strain (injury) , synonym (taxonomy) , genus , gene , genomic dna , ribosomal rna , bacteria , botany , microbiology and biotechnology , genetics , anatomy , gene expression
A taxonomic identification using a polyphasic approach was performed on strain NBS58-1 T , which was isolated from the interfacial sediment of Taihu Lake in China. Strain NBS58-1 T was Gram-stain-negative, aerobic, non-spore-forming and catalase-positive. Phylogenetic analyses based on 16S rRNA gene and three housekeeping genes ( rpoB , gyrB and dnaK ) sequences supported the position that strain NBS58-1 T should be classified within the genus Rufibacter . The 16S rRNA gene sequence of strain NBS58-1 T possessed the highest similarity to Rufibacter sediminis H-1 T (96.60 %), followed by Rufibacter glacialis MDT1-10-3 T (96.17 %). And the ANI value between strain NBS58-1 T and R. glacialis MDT1-10-3 T was 79.3 %. The respiratory quinone was menaquinone 7 (MK-7). The major cellular fatty acids comprised iso-C 15 : 0 and summed feature 3. Phosphatidylethanolamine, two unidentified phospholipids and four unidentified lipids were the main polar lipids. The genomic DNA G+C content was 51.3 mol%. Based on phenotypic features and phylogenetic position, a novel species with the name Rufibacter hautae sp. nov. is proposed. The type strain is NBS58-1 T =(KACC 21309 T =MCCC 1K04037 T ). We also proposed Rufibacter quisquiliarum as a latter heterotypic synonym of Rufibacter ruber .