Open AccessRufibacter latericius sp. nov., isolated from Baiyang Lake
Author(s) -
Yifan Yang,
Jifeng Chen,
WanRu Huang,
Ranran Zhang,
ShuangJiang Liu,
Chao Wang,
Jie Feng
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004500
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , halophile , bacteria , gene , botany , microbiology and biotechnology , genetics , anatomy
A novel Gram-stain-negative, strictly aerobic, rod-shaped, brick red-pigmented bacterium, designated R-22-1 c-1 T , was isolated from water from Baiyang Lake, Hebei Province, PR China. The strain was able to grow at 20–30 °C (optimum, 30 °C) and pH 6–7 (optimum, pH 6) in Reasoner’s 2A medium. 16S rRNA gene sequence and phylogenetic analyses of R-22-1 c-1 T revealed closest relationships to Rufibacter immobilis MCC P1 T (97.8 %), Rufibacter sediminis H-1 T (97.9 %) and Rufibacter glacialis MDT1-10-3 T (97.0 %), with other species of the genus Rufibacter showing less than 97.0 % sequence similarity. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids. The major cellular fatty acids were iso-C 15 : 0 , C 15 : 1 ω 6 c , C 17 : 1 ω 6 c , anteiso-C 15 : 0 , summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and summed feature 4 (iso-C 17 : 1 I and/or anteiso-C 17 : 1 B). The respiratory quinone was MK-7. The draft genome of R-22-1 c-1 T was 5.6 Mbp in size, with a G+C content of 50.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between strain R-22-1 c-1 T and related type strains were R. immobilis MCC P1 T (77.2 and 21.8 %), R. sediminis H-1 T (81.6 and 21.4 %) and R. tibetensis 1351 T (78.5 and 22.9 %). Based on these phylogenetic, chemotaxonomic and genotypic results, strain R-22-1 c-1 T represents a novel species in the genus Rufibacter , for which the name Rufibacter latericius sp. nov. is proposed. The type strain is R-22-1 c-1 T (=CGMCC 1.13570 T =KCTC 62781 T ).