Open AccessNovosphingobium aquimarinum sp. nov., isolated from seawater
Author(s) -
Ve Van Le,
So-Ra Ko,
SangAh Lee,
Long Jin,
ChiYong Ahn,
HeeMock Oh
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004493
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , microbiology and biotechnology , phylogenetics , bacteroidetes , bacteria , gene , biochemistry , genetics , anatomy
A novel Gram-stain-negative, aerobic, and rod-shaped bacterial strain, M24A2M T , was isolated from seawater in the Republic of Korea. On the basis of the 16S rRNA gene phylogeny, strain M24A2M T was found to be closely related to Novosphingobium pentaromativorans US6-1 T and Novosphingobium mathurense SM117 T with pair-wise sequence similarities of 97.4 and 96.9 %, respectively. Phylogenetic analysis of 16S rRNA sequences indicated that M24A2M T formed a branch with Novosphingobium pentaromativorans US6-1 T and represented a member of the genus Novosphingobium . The predominant cellular fatty acids were C 14 : 0 2-OH, summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ), and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ). The polar lipids of strain M24A2M T consisted mainly of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content was 63.9 %. Given the phenotypic characteristics along with the phylogenetic distinctness and chemotaxonomic features, strain M24A2M T is considered to represent a novel species within the genus Novosphingobium , for which the name Novosphingobium aquimarinum sp. nov. is proposed. The type strain of Novosphingobium aquimarinum sp. nov. is M24A2M T (=KCTC 72894 T =JCM 33983 T ).