Open AccessAgromyces kandeliae sp. nov., isolated from rhizosphere soil of Kandelia candel in a mangrove
Author(s) -
Ruijun Wang,
Yanghui Ye,
Yanfen Huang,
Yan-Fang Nie,
Shuai-Bo Han,
Xiaoyun Yu,
Choufei Wu,
Li Yang,
Min Wu,
Liqin Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004492
Subject(s) - kandelia candel , biology , 16s ribosomal rna , rhizosphere , strain (injury) , genomic dna , bacteria , botany , microbiology and biotechnology , phylogenetic tree , mangrove , biochemistry , dna , gene , genetics , ecology , anatomy
A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22 T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China. Strain Q22 T was able to grow at 10–40 °C (optimum 30 °C), pH 5.5–9.0 (optimum 7.0–8.0) and with 0–5.0% (w/v) NaCl (optimum 1.0 %). The genomic DNA G+C content was 71.9%. The average nucleotide identity, and in silico DNA–DNA hybridization values between strain Q22 T and the reference strains were 79.7–88.9% and 22.6–37.4%, respectively. The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteis o-C 15:0 , iso -C 16:0 and anteiso -C 17:0 . The major polar lipids of strain Q22 T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids. The strain Q22 T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans. The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22 T represents a novel species of the genus Agromyces , for which the name Agromyces kandeliae sp. nov. is proposed. The type strain is Q22 T (=MCCC 1K03340 T = KCTC 39961 T ).