Open AccessGramella bathymodioli sp. nov., isolated from a mussel inhabiting a hydrothermal field in the Okinawa Trough
Author(s) -
Lijun Liu,
Shuang Wang,
Shun Zhou,
Wentao Sun,
Tianyu Fu,
Yulin Zhang,
Xiaohua Zhang,
Min Yu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004488
Subject(s) - biology , 16s ribosomal rna , strain (injury) , bacteroidetes , bacteria , mussel , hydrothermal vent , genus , microbiology and biotechnology , botany , hydrothermal circulation , ecology , genetics , anatomy , paleontology
A Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped and non-flagellated marine bacterium strain, designated BOM4 T , was isolated from a mussel inhabiting the Tangyin hydrothermal field of the Okinawa Trough. The growth temperature was in the range of 16–40 °C, and the optimum temperature was 37 °C. Optimal growth occurred at pH 7.0 and in the presence of 1 % (w/v) NaCl. The predominant isoprenoid quinone of strain BOM4 T was identified as menaquinone-6 (MK-6). The predominant fatty acids (>10 %) were iso-C 15 : 0 (43.8 %) and iso-C 17 : 0 3-OH (17.5 %). The major polar lipids comprised one phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene sequence analyses, strain BOM4 T was found to be most closely related to Gramella aestuarii JCM 17790 T (96.7 %), followed by Gramella flava JLT2011 T (96.1 %), Gramella sediminilitoris GHTF-27 T (95.6 %) and Gramella gaetbulicola RA5-111 T (95.5 %) and with lower sequence similarities (93.7- 95.4 %) to other species of the genus Gramella . Genome relatedness between strain BOM4 T and G. aestuarii JCM 17790 T was computed using both average nucleotide identity and DNA–DNA hybridization with values of 75.6 and 19.3±2.4 %, respectively. The DNA G+C content of strain BOM4 T was 41.4 mol%. On the basis of polyphasic analysis, strain BOM4 T was considered to represent a novel species of the genus Gramella , for which the name Gramella bathymodioli sp. nov. is proposed. The type strain is BOM4 T (=MCCC 1K03735 T =JCM 33424 T ).