Leeia aquatica sp. nov., isolated from freshwater

A Gram-stain-negative, rod-shaped, obligately aerobic, motile by a single polar flagellum, chemoheterotrophic bacterium, designated strain IMCC25680 T , was isolated from surface water in Chungju Lake, Republic of Korea. 16S rRNA gene sequence analysis revealed that strain IMCC25680 T was most closely related to Leeia oryzae HW7 T with 95.5% sequence similarity and formed a robust clade with L. oryzae HW7 T . Whole genome sequencing showed that strain IMCC25680 T had a genome 3.6 Mbp long with 60.7 mol% DNA G+C content. Average nucleotide identity and digital DNA–DNA hybridization values between strain IMCC25680 T and L. oryzae HW7 T were 72.4% and 18.5%, respectively, indicating that the novel strain represents a novel species of the genus Leeia . The major cellular fatty acids of strain IMCC25680 T were iso-C 16:0 and summed feature 3 (comprising C 16:1 ω 6 c and/or C 16:1 ω 7 c ). The respiratory quinone detected in the strain was ubiquinone-8. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. On the basis of the phylogenetic and phenotypic characterization, strain IMCC25680 T was considered to represent a novel species within the genus Leeia , for which the name Leeia aquatica sp. nov. is proposed. The type strain is IMCC25680 T (=KACC 19487 T =NBRC 113132 T ).