Open AccessPseudomonas xionganensis sp. nov., isolated from Baiyangdian Lake in Xiong'an New Area
Author(s) -
Yingying Zhao,
Yifan Yang,
Fei He,
Chao Wang,
Jie Feng,
Xiumin Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004485
Subject(s) - biology , pseudomonas putida , 16s ribosomal rna , pseudomonas , phylogenetic tree , strain (injury) , microbiology and biotechnology , bacteria , phylogenetics , gene , genetics , anatomy
An aerobic, Gram-stain-negative, rod-shaped bacterium, designated as strain R-22–3 w-18 T , was isolated from Baiyangdian Lake in Xiong'an New Area, PR China. Growth was observed at 15–37 °C (optimum, 30 °C) and pH 7.0–10.0 (optimum, pH 8.0). 16S rRNA gene sequence analysis placed the strain in a separate branch in the Pseudomonas putida group, with Pseudomonas anguilliseptica DSM12111 T as its closest phylogenetic relative (98.2 %). Whole-genome comparisons, using average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) analyses, confirmed low genome relatedness to the closely related Pseudomonas species [below the recommended thresholds of 95 % (ANI), 95 % (AAI) and 70 % (DDH) for species delineation]. Phenotypic characterization tests could also distinguish this strain from other related species of the genus Pseudomonas . Therefore, based on genetic and phenotypic evidence, it is clear that strain R-22–3 w-18 T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas xionganensis sp. nov. is proposed. The type strain is R-22–3 w-18 T (=CGMCC 1.17250 T =KCTC 72658 T ).