Open AccessBrevibacterium profundi sp. nov., isolated from deep-sea sediment of the Western Pacific Ocean
Author(s) -
Shengxiang Pei,
Xie F,
Siwen Niu,
Lingqi Ma,
Rui Zhang,
Gaiyun Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004483
Subject(s) - biology , diamino acid , peptidoglycan , brevibacterium , 16s ribosomal rna , strain (injury) , phylogenetic tree , microbiology and biotechnology , diaminopimelic acid , phosphatidylglycerol , botany , bacteria , gene , cell wall , genetics , microorganism , gene sequence , phospholipid , anatomy , membrane , phosphatidylcholine
A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1 T , was isolated from a deep-sea sediment of the Western Pacific Ocean. Strain O1 T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1–5 % (w/v) NaCl. The predominant menaquinone was MK-8 (H 2 ), and anteiso-C 15 : 0 and anteiso-C 17 : 0 were the major fatty acids. The diagnostic diamino acid in the cell-wall peptidoglycan was meso -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid. The DNA G+C content of strain O1 T was 64.9 mol% and the genome size was 4.17 Mb. Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1 T belonged to the genus Brevibacterium . The values of average nucleotide identity and in silico DNA–DNA hybridization between strain O1 T and its close relatives were well below the thresholds used for the delineation of a new species. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1 T represents a novel species of the genus Brevibacterium , for which the name Brevibacterium profundi sp. nov. is proposed. The type strain is O1 T (=JCM 33845 T =MCCC 1A16744 T ).