Open AccessStreptococcus catagoni sp. nov., isolated from the respiratory tract of diseased Chacoan peccaries (Catagonus wagneri)
Author(s) -
Kristin Mühldorfer,
Claudia A. Szentiks,
Gudrun Wibbelt,
Mark van der Linden,
Christa Ewers,
Torsten Semmler,
Valerij Akimkin,
Jochen Blom,
Jörg Rau,
Tobias Eisenberg
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004471
Subject(s) - biology , microbiology and biotechnology , streptococcus , whole genome sequencing , 16s ribosomal rna , streptococcus equi , streptococcus iniae , genome , genetics , gene , bacteria
Novel catalase-negative, Gram-stain-positive, beta-haemolytic, coccus-shaped organisms were isolated from Chacoan peccaries that died from respiratory disease. The initial API 20 Strep profiles suggested Streptococcus agalactiae with acceptable identification scores, but the 16S rRNA gene similarity (1548 bp) to available sequences of streptococci was below 98 %. Next taxa of the genus Streptococcus , displaying highest similarities to the strains from this study, were S. bovimastitidis NZ1587 T (97.5 %), S. iniae ATCC 29178 T (97.5 %), S. hongkongensis HKU30 T (97.4 %), S. parauberis DSM 6631 T (97.1 %), S. penaeicida CAIM 1838 T (97.1 %), S. pseudoporcinus DSM 18513 T (97.0 %), S. didelphis DSM 15616 T (96.6 %), S. ictaluri 707-05 T (96.6 %), S. uberis JCM 5709 T (96.5 %) and S. porcinus NCTC 10999 T (96.4 %). All other Streptococcus species had sequence similarities of below 96.4 %. A sodA gene as well as whole genome-based core genome phylogeny of three representative strains and 145 available Streptococcus genomes confirmed the unique taxonomic position. Interstrain average nucleotide identity (ANI) and amino acid identity (AAI) values were high (ANI >96 %; AAI 100%), but for other streptococci clearly below the proposed species boundary of 95–96 % (ANI 90 %) between the novel strains, but well below the species boundary of 70 % for closely related Streptococcus type strains (23.5–19.7 %). Phenotypic properties as obtained from extended biochemical profiles and MALDI-TOF mass spectrometry supported the outstanding rank. Based on the presented molecular and physiological data of the six strains, we propose a novel taxon for which we suggest the name Streptococcus catagoni sp. nov. with the type strain 99-1/2017 T (=DSM 110457 T =CCUG 74072 T ) and five reference strains.