Open AccessPseudonocardia acidicola sp. nov., a novel actinomycete isolated from peat swamp forest soil
Author(s) -
Chollachai Klaysubun,
Kenika Lipun,
Kannika Duangmal
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004459
Subject(s) - biology , 16s ribosomal rna , arabinose , strain (injury) , diaminopimelic acid , botany , soil microbiology , microbiology and biotechnology , bacteria , biochemistry , xylose , gene , fermentation , genetics , anatomy
A novel actinobacterium, designated strain K10HN5 T , was isolated from a peat soil sample collected from Kantulee peat swamp forest, Surat Thani Province, Thailand and its taxonomic position was determined using a polyphasic approach. Strain K10HN5 T contained meso -diaminopimelic acid, arabinose, galactose, glucose and ribose in its whole-cell hydrolysates. The predominant menaquinone was MK-8(H 4 ). The major fatty acids were iso-C 16 : 0 , iso-C 15 : 0 and iso-C 16 : 1 H. Mycolic acids were not present. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, hydroxyphosphatidylmethylethanolamine and phosphatidylinositol. The 16S rRNA gene sequence analysis indicated that it was closely related to Pseudonocardia bannensis DSM 45300 T (97.9 %) and Pseudonocardia xinjiangensis JCM 11839 T (97.9 %). Strain K10HN5 T exhibited low average nucleotide identity and digital DNA–DNA hybridization values with P. bannensis DSM 45300 T (82.6, 28.7 %) and P. xinjiangensis JCM11839 T (76.3, 22.2 %). The DNA G+C content of strain K10HN5 T was 72.4 mol%. Based on polyphasic data, strain K10HN5 T represents a novel species of the genus Pseudonocardia , for which the name Pseudonocardia acidicola sp. nov. is proposed. The type strain is K10HN5 T (=TBRC 10048 T =NBRC 113897 T ).