Open AccessOceanipulchritudo coccoides gen. nov., sp. nov., isolated from marine sediment within the family Puniceicoccaceae
Author(s) -
Xi Feng,
Qi-Hang Zou,
Xiao-Yu Zhang,
Meng-Qi Ye,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004458
Subject(s) - biology , 16s ribosomal rna , genus , strain (injury) , coccus (insect) , genomic dna , botany , verrucomicrobia , microbiology and biotechnology , bacteroidetes , gene , genetics , anatomy
A Gram-stain-negative, aerobic coccus, designated CK1056 T , was isolated from coastal sediment of Xiaoshi Island, Weihai, PR China. Strain CK1056 T was found to grow at 15–37 °C (optimum, 30 °C), with 0.5–6.5 % (w/v) NaCl (optimum, 3.5 %) and displayed alkaliphilic growth within the pH range of pH 6.5–10.0 (optimum, pH 8.0). The major fatty acids identified were iso-C 15 : 0 and summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ). The main polar lipids consisted of aminophosphoglycolipid and phosphatidylethanolamine. The predominant respiratory quinone was MK-7. The G+C content of the genomic DNA was 54.0 mol%. The result of the 16S rRNA gene sequence analysis confirmed the affiliation of this micro-organism to the family Puniceicoccaceae , with Coraliomargarita akajimensis KCTC 12865 T as its closest relative with only 88.0 % sequence similarity. From the taxonomic data obtained in this study, we propose that the new marine isolate be placed into a novel species within a novel genus in the family Puniceicoccaceae , phylum Verrucomicrobia , for which the name Oceanipulchritudo coccoides gen. nov., sp. nov. is proposed. The type strain is CK1056 T (=KCTC 72798 T =MCCC 1H00425 T ).