Open AccessRoseomonas algicola sp. nov., isolated from a green alga, Pediastrum duplex
Author(s) -
Hyung Min Kim,
Shehzad Abid Khan,
Dong Min Han,
Byung Hee Chun,
Che Ok Jeon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004454
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , ribosomal rna , bacteria , botany , strain (injury) , gene , genetics , anatomy
A Gram-stain-negative, strictly aerobic bacterium, designated strain PeD5 T , was isolated from a green alga Pediastrum duplex from the Nakdong river of the Republic of Korea. Cells were non-motile cocci, catalase-negative and oxidase-positive. Growth of PeD5 T was observed at 25–40 °C (optimum, 35 °C) and pH 5.0–10.0 (optimum, pH 7–8), and in the presence of 0–0.25% (w/v) NaCl (optimum, 0%). PeD5 T contained C 16:0 , C 18:1 ω7 c 11-methyl, summed feature 3 (comprising C 16:1 ω7 c and/or C 16:1 ω6 c ) and summed feature 8 (comprising C 18:1 ω7 c and/or C 18:1 ω6 c ) as major cellular fatty acids (>5%) and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and an unidentified aminolipid were detected as major polar lipids. The genomic DNA G+C content of PeD5 T was 71.0 mol%. PeD5 T was most closely related to Roseomonas stagni HS-69 T with a 97.6% 16S rRNA sequence similarity and shared less than 96.3% 16S rRNA sequence similarities with type strains of other species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that PeD5 T formed a phyletic lineage with Roseomonas stagni HS-69 T within the genus Roseomonas . On the basis of results of phenotypic, chemotaxonomic and molecular analysis, strain PeD5 T clearly represents a novel species of the genus Roseomonas , for which the name Roseomonas algicola sp. nov. is proposed. The type strain is PeD5 T (=KACC 19925 T =JCM 33309 T ).